NanoString nCounter data are gene expression assays where there is no need for the use of enzymes or amplification protocols and work with fluorescent barcodes (Geiss et al. (2018) <doi:10.1038/nbt1385>). Each barcode is assigned a messenger-RNA/micro-RNA (mRNA/miRNA) which after bonding with its target can be counted. As a result each count of a specific barcode represents the presence of its target mRNA/miRNA. 'NACHO' (NAnoString quality Control dasHbOard) is able to analyse the exported NanoString nCounter data and facilitates the user in performing a quality control. 'NACHO' does this by visualising quality control metrics, expression of control genes, principal components and sample specific size factors in an interactive web application.
Version: | 2.0.0 |
Depends: | R (≥ 3.6.0) |
Imports: | utils, data.table, ggplot2 (≥ 3.3.0), ggbeeswarm (≥ 0.6.0), ggforce (≥ 0.3.1), ggrepel (≥ 0.8.1), knitr (≥ 1.25), rmarkdown (≥ 1.16), shiny (≥ 1.4.0), shinyWidgets (≥ 0.4.9) |
Suggests: | roxygen2 (≥ 7.2.0), testthat (≥ 2.2.1), covr (≥ 3.3.2), Biobase, GEOquery, limma |
Published: | 2022-05-31 |
Author: | Mickaël Canouil [aut, cre], Roderick Slieker [aut], Gerard Bouland [aut] |
Maintainer: | Mickaël Canouil <mickael.canouil at cnrs.fr> |
BugReports: | https://github.com/mcanouil/NACHO/issues |
License: | GPL-3 |
URL: | https://github.com/mcanouil/NACHO/, https://m.canouil.fr/NACHO/ |
NeedsCompilation: | no |
SystemRequirements: | pandoc (>= 1.12.3), pandoc-citeproc |
Citation: | NACHO citation info |
Materials: | README NEWS |
CRAN checks: | NACHO results |
Reference manual: | NACHO.pdf |
Vignettes: |
NACHO-analysis NACHO |
Package source: | NACHO_2.0.0.tar.gz |
Windows binaries: | r-devel: NACHO_2.0.0.zip, r-release: NACHO_2.0.0.zip, r-oldrel: NACHO_2.0.0.zip |
macOS binaries: | r-release (arm64): NACHO_2.0.0.tgz, r-oldrel (arm64): NACHO_2.0.0.tgz, r-release (x86_64): NACHO_2.0.0.tgz, r-oldrel (x86_64): NACHO_2.0.0.tgz |
Old sources: | NACHO archive |
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